Figure 4

The lncRNA EVADR is partially derived from a MER48 ERV element. (a) Gene structure indicating the MER48 element overlapping with the 5′ termini of EVADR (red); lncRNA exons are shown in blue, predicted poly(A) signal in yellow and the promoter by a bent arrowhead. (b) Partial sequences of 10 clones from 5′ RNA ligase-mediated RACE analysis of MER48 initiated EVADR transcripts aligned to human genomic DNA. The predicted TATA box is indicated by a line, the minor transcriptional start sites by an asterisk, and the predominant initiating nucleotide is bolded and indicated by a bent arrow. The RNA adaptor sequences are light grey and in lower case. (c) Promoter deletion experimental design showing truncations of the MER48 element. The MER48 LTR is indicated by a red arrow, the luciferase ORF by green rectangles, and EVADR is indicated by blue rectangles. (d) Results of the promoter analysis in K562 cells. (e) Results of the promoter analysis in SW480 cells. *Adjusted P < 0.05; **adjusted P < 0.005; two-sample t-test with Bonferroni correction.