Table 2 Primer sequences
From: Activation of an endogenous retrovirus-associated long non-coding RNA in human adenocarcinoma
Oligo | Purpose | Sequence (5′-3′) |
|---|---|---|
oHL_0005 | Forward RT-PCR primers for EVADR transcript | TGATGCCATTTTCAGCCTCAG |
oHL_0006 | Reverse RT-PCR primers for EVADR transcript | TGGCCGCTCAGATTCTCTATC |
oHL_0011 | Clone MER48 LTR upstream of EVADR. XhoI/HindIII | GGCTCGAGTAAGGGAATGAATAACTCCG |
oHL_0012 | Clone MER48 LTR upstream of EVADR. XhoI/HindIII | GGCTCGAGATATGTACCCTGTGAAGACC |
oHL_0015 | Cloning small fragment of MER48 element | GGCTCGAGGTGCTGATGCCATTTTCAGCC |
oHL_0013 | Clone MER48 LTR upstream of EVADR. XhoI/HindIII | GGAACCTTACATGCTGTTTTAATGAGCG |
oHL_0016 | Same as oHL_0011 but reversed restriction site. XhoI | CCAAGGTTTAAGGGAATGAATAACTCCG |
oHL_0017 | Same as oHL_0012 but reversed restriction site. HindIII | GGCTCGAGACATGCTGTTTTAATGAGCG |
oHL_0014 | Same primer as oHL_0006 but for cloning 5′RACE products | CCGGATCCTGGCCGCTCAGATTCTCTATC |