Inferred origins of recurrent mutations and their VAFs. Sample color coding is as in Figure 2. Green bars, VAF of PIK3CA H1047R; blue bars, VAF of PIK3CA H1047L. Green or blue circles on branches mark the origin of the indicated number of mutations, where a plus sign indicates that there may be additional events that we cannot resolve. Orange bars and circles pertain to the T-to-C change in position 101,160,839 on chromosome 11 in patient 3.