Fig. 1

Applications of CRISPR/genome editing for precision cancer models. CRISPR has been used to generate genetically modified mouse models such as KO/KI germline models, somatic genome editing models and mouse models for drug treatment. CRISPR has been proved a useful tool to investigate chromosomal engineering, generate ex vivo leukemia models and identify drug resistance genes through genome editing of cell lines. CRISPR has also been used to correct disease-associated genes through homology-directed repair pathway. In combination with traditional Cre-LoxP system, CRISPR can generate conditional KO/KI mouse models and further the understanding of cancer progression. KO, knockout; KI, knock-in; sgRNA, single guide RNA