Fig. 4

Triglyceride profiles of mouse models associated with de novo lipogenesis. a O2PLS-DA analysis of the triglyceride profile of blood plasma from 4-month-old wild-type (wt) and ob/ob mice on either a chow or high fat diet (HFD). The first two OPLS-DA components are shown (n = 9 per group; R²(X) = 71.6%; R²(Y) = 94.8%; Q² = 89.2%; three O2PLS-DA components and two orthogonal components were fitted). b O2PLS-DA analysis of the triglyceride profile of blood plasma from wild-type and ob/ob mice on a chow diet. The first O2PLS-DA component and orthogonal component are shown (R²(X) = 71.8%; R²(Y) = 99.8%; Q² = 96.4%; one O2PLS-DA component and three orthogonal components were fitted). c Relative intensity of blood plasma TAG(50:1) in 4-month-old wild-type and ob/ob mice under high fat diet (HFD) and chow diet (n = 9 for each group; 2.1-fold in the ob/ob mice compared with the wild type on a chow diet (p = 0.0002)). d Increased expression of genes involved in DNL in the liver of ob/ob mice compared with wild-type controls as measured by RT-PCR. Expression of DNL genes in liver from 4-month-old wild-type control mice (n = 6) and ob/ob mice (n = 6). Data are represented as mean ± standard error of the mean and all data were normalized to 18S rRNA. Two-way ANOVA, p < 0.0001. FASN fatty acid synthase, ACACA acetyl-CoA carboxylase 1, SREBP1F sterol regulatory element binding protein-1c, SCD1 stearoyl-coenzyme A desaturase 1, GPAM glycerol-3-phosphate acyltransferase, mitochondrial. e O2PLS-DA analysis of the triglyceride profile of blood plasma from wild-type mice on a high fat diet (HFD; low de novo lipogenesis) and ob/ob mice on a chow diet (high de novo lipogenesis). f Relative intensity of blood plasma TAG(50:1) in 4-month-old wild-type mice on a high fat diet and ob/ob mice on a chow diet (n = 9 for each group). ***p < 0.001 according to Student’s t-test