Fig. 1

ALKBH3 localizes genome-wide at promoters. a High confident (MACS-called) ALKBH3 ChIP-seq peaks are enriched at promoters but are depleted from inter- and intragenic regions. b Heatmap of all (including non-MACS called) ALKBH3 bound promoters. Two major groups of ALKBH3 promoters (group I and group II), with substantial different binding properties, have been identified (see also Fig. 4a). c ALKBH3 profile at all bound promoters in comparison to ChIP-Seq with control IgG. d ALKBH3 shRNA #1 infected PC3 cells have reduced protein levels of ALKBH3, compared to control cells. e Immunofluorescence of ALKBH3 in U2OS cells infected with control shRNA or ALKBH3 shRNA #1 demonstrating the specificity of the ALKBH3 antibody. f High confidence peaks at three example genes (including the ALKBH3 gene itself). The ChIP-Seq results have been validated by ChIP qRT-PCR. The enrichment is strongly reduced in the knockdown cells. (* = P <0.05). See also Additional file 1: Figure S1