Fig. 3

ALKBH3 depletion induces an inflammatory response. a Volcano plot of the microarray data after ALKBH3 knockdown. A two-fold expression change and a P value <0.05 were used as cutoff. b Gene expression does not significantly change at ALKBH3 target genes. c Gene ontology analysis of upregulated genes showed strong induction of inflammatory genes. d qRT-PCR analysis of several interferon stimulated genes (ISGs) with two independent ALKBH3 shRNAs in comparison to ALKBH2 shRNA infected cells. The data were normalized via GAPDH to control shRNA infected cells. Error bars show standard deviation of triplicates. Significance was tested using a student’s t-test. P <0.05 has been considered as significant. e Heatmaps showing the expression change of affected interferon inducible genes (ISGs) and interleukins, at 48 h and 96 h post selection