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Fig. 5 | Genome Medicine

Fig. 5

From: The oxidative demethylase ALKBH3 marks hyperactive gene promoters in human cancer cells

Fig. 5

Activating factors are enriched at group I promoters. a Multiple factors were investigated to characterize group I promoters. For each feature the number of ChIP-Seq tags was counted per promoter (see Additional file 1: Figure S5). The mean tag number was used to examine whether or not a factor is enriched/depleted at group I promoters relative to group II promoters. X = Mean of group I promoters/Mean of all promoter. Y = Mean of group II promoters/Mean of all promoter. Many factors are stronger enriched at group I promoters than at group II promoters (at least 10 % more, green). b Six examples of highly enriched factors. The promoters in the heatmaps are sorted as in Fig. 1b (−1,500/+1,500 bp). The average profiles show a strong enrichment at group I promoters. c Hypothetical model of the group I promoter functioning. At group I promoters, transcription factors (purple) are clustered, which correlates with the presence of the Mediator complex and cohesin. P300 is recruited by transcription factors and Mediator and might facilitate H3K122 acetylation, which could lead to nucleosome eviction. Mediator (blue), together with TFIID (brown) and TFIIH (yellow) and possibly other factors might form a non-classical initiation complex, leading to stronger transcriptional activity. d At group II promoters the levels of regulatory factors is less, leading to classical initiation processes

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