Fig. 2

Genome-wide transcriptome profiling and cluster analysis uncovers functionally related groups of genes that are co-regulated as a function of passage and time. Genes with a two-fold or greater change in gene expression as a function of passage or time were segregated based on their expression profiles using AutoSOME 2.1. Individual clusters with similar expression profiles were placed in major expression groups (a-g) following manual examination. The headers of each group are representative of gene ontology classes that are over-represented in each group. The value associated with the ontology groups is the enrichment P value. The color scale indicates the expression level as a percentage of the maximum value for that gene. Each column represents a unique biological replicate (n = 3). Rows represent unique genes. The corresponding gene identifiers and associated data can be found in Additional file 3: Table S2