Fig. 3

Differential expression of a core set of genes whose products comprise a functional interactome is an underlying feature of chronically wounded RPE cells. The 565 genes with two-fold or greater differences in expression between 3-day P0 and P5 cultures were analyzed using STRING 9.1 to identify genes whose products have experimentally determined protein-protein interactions (Experimental) or are part of a common curated pathway (Knowledge) and the resulting interactome was rendered using Cytoscape 3.0.2. The interactome is segregated into two sub-modules, one enriched for gene products involved in the cell cycle and the other for genes that are often associated with the response to wound stimuli. The line color indicates the nature of the connection with direct protein-protein interactions taking precedence over common pathway. The gene node color represents the log₂ transformed P5:P0 gene expression ratio as determined by microarray analysis