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Fig. 4 | Genome Medicine

Fig. 4

From: Restoration of mesenchymal retinal pigmented epithelial cells by TGFβ pathway inhibitors: implications for age-related macular degeneration

Fig. 4

FGF2 or small molecule inhibitors of wound response promote differentiation at low plating density. Passage 0 cells were plated at 4,000 cells/cm2 and grown in the presence of 500 nM A-83-01, 2 ng/mL FGF2, 500 nM thiazovivin, 200 nM LDN-193189, or 5 μM XAV939. The vehicle (DMSO) alone served as the control. a Whole culture images (above), phase contrast (Ph) and brightfield micrographs (Bf) taken at day 32 demonstrate that A-83-01, FGF2, and thiazovivin treated cultures maintain the capacity to differentiate after low density plating. b Whole transcriptome profiles of the 32-day treated and control low density P0 cultures were determined and compared to the expression profiles of differentiated 32-day high density P0 cultures. The transcriptome profiles of 32-day differentiated (P0) and undifferentiated (P5) RPE cells plated at 80,000 cells/cm2 from three donors are shown for comparison. Only genes with two-fold or greater P0:P5 expression ratios and a P0 or P5 net intensity ≥15 were evaluated. The color scale indicates the log2 transformed expression level relative to the mean value of the 32-day high density P0 cells. Black indicates identity. The values on the right indicate the Pearson’s product–moment correlation coefficient (PC) for all expressed genes relative to the mean of the high density P0 profiles

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