Fig. 6

A-83-01 treatment largely restores the RPE transcriptome. Untreated P0 cells, untreated P4 and P4 cells treated with 500 nM A-83-01 were seeded at 80,000 cells/cm². After 32 days the cells were harvested and their transcriptome profiles were determined using RNA-Seq. a Heatmap showing the expression levels relative to the mean of the three P0 cultures. Only the 4,590 genes with significant (FDR ≤0.01) expression differences between P0 and untreated P4 cells are depicted. The values on the right indicate the Pearson’s product–moment correlation coefficient (PC) for all expressed genes relative to the mean of the high density P0 profiles. b Relative expression levels of select genes with well-known roles in RPE-specific functions or EMT and wound response. For each gene, the expression levels are normalized relative to the maximum value (mean +/− SEM, n = 3)