Fig. 5

A representative clinical example of how NGS is utilized in recurrent lung adenocarcinoma. The illustrative case from the text has been fitted to the outline in Fig. 1. In a lung adenocarcinoma, there are a number of actionable mutations; this case shows a canonical EGFR mutation, treated with erlotinib. There are actually now two levels of resistance that can develop, illustrated in rows 3 and 4. FFPE formalin-fixed, paraffin-embedded specimen