Fig. 6

Psoriasis-specific and non-specific DEGPs have divergent cytokine responses (stratified GSEA). a Cytokine treatments that differentially impact expression of psoriasis-specific and non-specific DEGPs (cultured KCs; *HaCaT cells; **reconstituted epidermis). The 153 PP-increased DEGPs were ranked according to the percentage of skin diseases in which the mRNA’s expression was correspondingly increased. Sliding DEGP windows (10 DEGPs/window) were analyzed to assess enrichment of DEGPs among genes induced by the cytokine treatment (red indicates windows with cytokine-induced DEGPs; blue indicates windows with cytokine-repressed DEGPs). Robust regression was used to quantify the left-to-right trend in enrichment statistics, and listed experiments were associated with the most negative (top) or positive (bottom) enrichment statistic trends (out of 59 experiments screened). The concentration, treatment duration, and GEO series identifier is listed for each cytokine treatment. Enrichment statistics and p values were also calculated using the complete set of 153 DEGPs (right margin; red/blue font indicates FDR < 0.05, Wilcoxon rank sum test). b The 25 most psoriasis-specific PP-increased DEGPs are enriched among genes induced by IL17A (p < 0.001; GSE52361). Genes were ranked along the horizontal axis according to how strongly they are induced by IL17A (left/red, IL17A-induced; right/blue, IL17A-repressed). The figure shows cumulative overlap between the 25 psoriasis-specific DEGPs and this ranked gene list. c The 25 most non-specific PP-increased DEGPs are not enriched among genes induced by IL17A (p = 0.619; GSE52361). Genes were ranked along the horizontal axis according to how strongly they are induced by IL17A (left/red, IL17A-induced; right/blue, IL17A-repressed). The figure shows cumulative overlap between the 25 non-specific DEGPs and this ranked gene list