|
Genetic annotations*
|
IOW: atopy statusa
|
IOW: high IgEb
|
BAMSE: atopy statusc
|
|---|
|
CpG Site
|
Chr
|
Gene name
|
CpG location
|
Adj. OR
|
P-Value
|
Adj. OR
|
P-value
|
Adj. OR
|
P-value
|
|---|
|
cg04983687
|
16
|
ZFPM1
|
Body
|
0.239
|
6.46E−09
|
0.158
|
3.54E−05
|
0.269
|
3.17E−06
|
|
cg09249800
|
1
|
ACOT7
|
Body
|
0.239
|
8.52E−09
|
0.141
|
1.13E−05
|
–
|
–
|
|
cg12819873
|
11
|
PRG2
|
5′UTR
|
0.065
|
1.38E−08
|
0.045
|
7.37E−05
|
0.140
|
2.36E−05
|
|
cg07908654
|
13
|
–
|
–
|
0.145
|
1.14E−07
|
0.103
|
8.44E−05
|
0.206
|
2.21E−04
|
|
cg06824199
|
1
|
KIAA0494
|
Body
|
0.107
|
6.28E−07
|
0.073
|
1.93E−04
|
0.118
|
2.96E−06
|
|
cg27469152
|
17
|
EPX
|
3′UTR
|
0.091
|
1.34E−06
|
0.025
|
6.06E−05
|
0.099
|
1.00E−05
|
|
cg27468224
|
4
|
–
|
–
|
0.123
|
1.62E−06
|
0.073
|
5.01E−04
|
0.262
|
1.89E−02
|
|
cg13233042
|
11
|
ATL3
|
Body
|
0.192
|
5.06E−06
|
0.105
|
1.82E−04
|
0.194
|
9.75E−05
|
|
cg13197551
|
20
|
LSM14B
|
3′UTR
|
0.160
|
5.67E−06
|
0.094
|
5.64E−04
|
0.312
|
1.04E−02
|
|
cg07765167
|
17
|
MRPL45
|
TSS1500
|
0.087
|
6.50E−06
|
0.043
|
5.96E−04
|
–
|
–
|
|
cg09332506
|
1
|
COPA
|
Body
|
0.123
|
7.77E−06
|
0.126
|
3.62E−03
|
0.196
|
8.72E−05
|
|
cg17041511
|
17
|
–
|
–
|
0.143
|
1.27E−05
|
0.075
|
8.14E−04
|
0.133
|
4.31E−05
|
|
cg24836822
|
7
|
KCNH2
|
Body
|
0.311
|
1.36E−05
|
0.225
|
7.13E−04
|
–
|
–
|
|
cg07970948
|
7
|
ZNF862
|
Body
|
0.243
|
1.38E−05
|
0.109
|
2.55E−04
|
0.313
|
1.61E−05
|
|
cg25854298
|
10
|
ASCC1
|
Body
|
0.221
|
3.69E−05
|
0.144
|
1.24E−03
|
0.149
|
1.21E−06
|
|
cg09635874
|
13
|
FARP1
|
Body
|
8.084
|
4.89E−05
|
2.591
|
2.44E−01
|
1.149
|
8.59E−01
|
|
cg04085542
|
5
|
FAM172A
|
Body; 5′UTR
|
2.663
|
7.99E−05
|
1.250
|
5.48E−01
|
0.936
|
7.93E−01
|
|
cg03553407
|
4
|
ARHGAP10
|
Body
|
4.330
|
1.63E−04
|
2.411
|
1.92E−01
|
2.010
|
2.16E−01
|
|
cg12819826
|
19
|
PPAN; PPAN-P2RY11
|
TSS1500
|
0.213
|
2.45E−04
|
0.229
|
4.02E−02
|
1.788
|
2.87E−01
|
|
cg00854799
|
1
|
PEX10; RER1
|
3′UTR
|
4.209
|
2.48E−04
|
2.930
|
8.27E−02
|
1.464
|
5.18E−01
|
|
cg05652668
|
7
|
LUC7L2
|
1st exon; 5′UTR
|
0.280
|
3.07E−04
|
0.517
|
2.39E−01
|
0.820
|
7.35E−01
|
|
cg17971837
|
22
|
PVALB
|
TSS1500
|
0.122
|
7.90E−04
|
0.102
|
4.11E−02
|
0.188
|
2.60E−02
|
- Associations for methylation M-values with atopy and high IgE from the IOW epigenetic sample, as well as with atopy in the BAMSE replication sample. IOW analyses were adjusted for predicted cell proportions of CD8+ T cells, CD4+ T cells, natural killer cells, B cells, monocytes, granulocytes, and sex. BAMSE replication analyses were adjusted for the same predicted cell proportions, sex, batch (bisulfite treatment date), and asthma treatment
-
Abbreviations: Adj. OR adjusted odds ratio, BAMSE Children, Allergy, Milieu, Stockholm, Epidemiology cohort, Chr Chromosome number, CpG cytosine–phosphate–guanine, IgE Immunoglobulin E, IOW Isle of Wight cohort, TSS Transcription Start Site, UTR untranslated region
-
*CpGs and their annotations highlighted in bold were the sites replicated in the BAMSE cohort
-
aAtopy defined as at least one positive skin prick test; significant sites determined via α = 8.06E−4 (0.05/62 tests)
-
bHigh IgE defined as serum IgE ≥ 200 kU/L; significant sites determined via α = 8.06E−4 (0.05/62 tests)
-
cAtopy defined as serum IgE antibody ≥ 0.35 kUA/L, to any allergen; significant sites determined via α = 0.05
