Fig. 8

A focus gene analysis independent of COO class verifies the dominant polarized immune response in DLBCL. a An outline of the focus gene analysis (high resolution version in Additional file 3). Upper panel: the approach within each data set, with initial selection of the 80 % most variable genes, and subsequent generation of linked matrices of gene correlation values and associated p values. Middle panel: the merging of all data sets (11 data sets; data set GSE10846 subdivided by treatment type) is shown to give matrices of median correlations and p values. Lower panel: the selection of an individual focus gene for downstream analysis. b Results of integrated gene signature and ontology analysis for two focus genes (left panel TRAT1) and (right panel FGL2) displaying the clustering of enriched signature and GO terms. General terms corresponding to major correlation clusters are illustrated to the right of each heatmap. Corresponding high resolution versions are available in Additional files 18 and 19, which also include the corresponding heatmaps clustered from the gene perspective