Fig. 4

Metagenomic identification of HCV from a clinical serum sample by nanopore sequencing. a Time line of sequencing runs on flow cell #2 with HepC1 sample reloading, plotted as a function of elapsed time in hours since the start of flow cell sequencing. b Cumulative number of all sequenced reads (black line) and HCV viral reads (red line), plotted as a function of individual sequencing run time in minutes. c Taxonomic donut charts generated using the MetaPORE bioinformatics analysis pipeline. The total number of reads analyzed is shown in the center of the donut. d Coverage and pairwise identity plots generated in MetaPORE by mapping reads aligning to HCV to the closest matching reference genome. Data were analyzed in MetaPORE on a 64-core Ubuntu Linux server using the January 2015 NT reference database