Fig. 2

PCR amplification of the Balamuthia rps3 mitochondrial gene. The variable length of the rps3 intron among eight different Balamuthia strains (seven newly sequenced mitochondrial genomes and the case patient) suggests that this gene may be an attractive target for development of a molecular genotyping assay. Lane 8 corresponds to the DNA ladder (faint appearance), while lanes 3 and 4 correspond to an additional clinical Balamuthia isolate whose mitochondrial genome was not sequenced