Fig. 8

Effect of immunization with 09 MIV on the repertoire of peripheral B-lymphocytes with homo- and heterosubtypic seroconversion. Changes in the relative frequency of use of IGHV segments (Δ day 7 – day 0) for individuals with homotypic seroconversion (n = 7) (a, c), and with heterosubtypic seroconversion (n = 4) (b, d) for 47 IGHV segments. IGHV usage is expressed as relative transcription (unclustered reads) (a, b), and as the proportion of clonotypes using a particular IGHV segment (c, d). The dotted line indicates three standard deviations, which corresponds to 18.5 for relative transcription and 10.3 for clonotypic frequency above the means, 0.0014 and 0.003, respectively. Arrows indicate clonotypes selected for experimental validation. IGHV segments in bold letters indicate relevant expansions