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Table 1 Sequencing metrics of the analyzed VH sequences

From: Longitudinal analysis of the peripheral B cell repertoire reveals unique effects of immunization with a new influenza virus strain

Sequencing results Parameter Totalsa Amplicon library (Average ± SD)b
Raw output 454 reads 1,792,504 40,738 ± 27,496
Total bases (bp) 778,487,667 17,692,901 ± 12,073,169
Average length (bp) ND 483 ± 77
Quality and size filters Eliminated reads (%) 27 % 24 % ± 14
Sequences included in the analysis Analyzed with ImmunediveRsity 1,302,865 29,610 ± 18,114
Clonotypes 71,568 1,626 ± 938
Lineages 218,910 4,975 ± 3,378
  1. aTotal parameters of five 454-Roche Titanium sequencing runs. Forty-four gene libraries were generated from 13 individuals
  2. bEach gene library corresponds to one of six conditions: day 0 and day 7 after vaccination with TIV2008/2009, day 0 and day 7 after vaccination with 09 MIV2009, and day 0 and day 7 after vaccination with TIV2014/2015