|
Sequencing results
|
Parameter
|
Totalsa
|
Amplicon library (Average ± SD)b
|
|---|
|
Raw output
|
454 reads
|
1,792,504
|
40,738 ± 27,496
|
|
Total bases (bp)
|
778,487,667
|
17,692,901 ± 12,073,169
|
|
Average length (bp)
|
ND
|
483 ± 77
|
|
Quality and size filters
|
Eliminated reads (%)
|
27 %
|
24 % ± 14
|
|
Sequences included in the analysis
|
Analyzed with ImmunediveRsity
|
1,302,865
|
29,610 ± 18,114
|
|
Clonotypes
|
71,568
|
1,626 ± 938
|
|
Lineages
|
218,910
|
4,975 ± 3,378
|
-
aTotal parameters of five 454-Roche Titanium sequencing runs. Forty-four gene libraries were generated from 13 individuals
-
bEach gene library corresponds to one of six conditions: day 0 and day 7 after vaccination with TIV2008/2009, day 0 and day 7 after vaccination with 09 MIV2009, and day 0 and day 7 after vaccination with TIV2014/2015
