Fig. 6
Non-canonical as well as canonical subunits inhibit apoptosis of HL cells. a HL cell lines (L1236, L540 and KM-H2) were treated with two distinct siRNA sequences against p50/RelA and p52/RelB, as indicated and harvested 3 days after the end of the siRNA treatments. Overall redox activity of the cells was measured using Alamar Blue assay. Error bars represent SEM (n = 3). See Additional file 2: Figures S6A and B for time-course experiments. For time-course experiments see Additional file 2: Figures S6A and B. b WB analysis of p50/RelA or p52/RelB KD efficiencies and expression of the initiator caspase 8 (p18), the initiator caspase 9 (p10), and the effector caspase 3 (p17) in the samples of L1236 cells treated with siRNAs as described above. Protein levels of the two apoptosis inhibitors (c-FLIPS/L and Bcl-XL) identified as NF-κB target genes are also shown. α-tubulin was used as loading control