Fig. 1
From: Ultra-deep and quantitative saliva proteome reveals dynamics of the oral microbiome
Workflow for ultra-deep and quantitative saliva proteomics. (1, 2) Saliva is collected with a sterile cotton swab and its proteins are denatured, digested, and purified according to the iST protocol [12]. (3) Depending on the desired proteome depth, samples are either separated into eight fractions or directly measured in single runs. (4) Peptides are measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS). (5) MaxQuant identifies and quantifies the proteins and enables statistical analysis in the Perseus software environment. The required time for each of the steps is indicated below each panel