Fig. 3

IL-4Rα and Stat6-dependency of IL-4-regulated miRNA expression in mouse macrophages. a miR-342-3p, miR-99b, and miR-125a-5p expression in IL-4-stimulated or unstimulated wild-type (WT) and IL-4Rα-defficient (IL-4Rα KO) mouse bone marrow-derived macrophages as measured by stem-loop RT-qPCR. Each data point represents the mean and standard deviation of four or three (WT or IL-4Rα KO, respectively) individual animals. *P ˂ 0.05, **P ˂ 0.01, ***P ˂ 0.001. b The number of miRNAs showing significant (FDR ˂0.1) IL-4-dependent regulation in wild-type (WT) and Stat6-deficient (STAT6 KO) macrophages. c Stem-loop RT-qPCR-based quantification of miR-342-3p, miR-99b, and miR-125a-5p expression in IL-4-stimulated or unstimulated WT and Stat6 KO mouse bone marrow-derived macrophages. Each data point represents the mean and standard deviation of three individual animals. *P ˂ 0.05, **P ˂ 0.01, ***P ˂ 0.001