Fig. 4

Mechanism of IL-4-dependent co-regulation of miR-342-3p and EVL expression in human and mouse macrophages. a Strand-specific GRO-Seq, CTCF, and Rad21-specific ChIP-seq signals in unstimulated as well as H3K4m3, H3K4m1, H3K27Ac, Pu.1, and Stat6 ChIP-Seq signals in IL-4-stimulated and unstimulated mouse macrophages at the EVL locus visualized by the Integrative Genomics Viewer. b RT-qPCR-based measurement of Evl expression in bone marrow (BM) cells and IL-4 stimulated or unstimulated bone marrow-derived macrophages (BMDM) in wild-type (WT) or Stat6-deficient (Stat6 KO) mice. Each data point represents the mean and standard deviation (SD) of three individual animals. **P ˂ 0.01. c Stem-loop RT-qPCR-based quantification of miR-342-3p expression in bone marrow cells and IL-4 stimulated or unstimulated bone marrow-derived macrophages in WT or Stat6 KO mice. Each data point represents the mean and SD of three individual animals. **P ˂ 0.01. d IL-4-induced Stat6 binding of two regulatory regions of the mouse EVL gene in WT and Stat6 KO mouse macrophages measured by ChIP-qPCR. Columns represent mean arbitrary units described in the “Methods” section ± SD. e RT-qPCR-based measurement of EVL expression during human macrophage differentiation in the absence or presence of IL-4 (a representative example of three independent human donors is shown). f Stem-loop RT-qPCR-based quantification of miR-342-3p expression during human macrophage differentiation in the absence or presence of IL-4 (a representative example of three independent human donors is shown). g IL-4-induced recruitment of STAT6 to the human EVL (hEVL) locus in macrophages obtained from two independent human donors (D1 and D2) measured by ChIP-qPCR. Data are expressed as mean ± SD