Fig. 5

IL-4-dependent and Stat6-mediated repression of the miR-99b-125a miRNA polycistron. a Strand-specific GRO-Seq, CTCF, and Rad21-specific ChIP-seq signals in unstimulated as well as H3K4m3, H3K4m1, H3K27Ac, Pu.1, and Stat6 ChIP-Seq signals in IL-4-stimulated and unstimulated mouse macrophages at the miR-99b-125a and Spaca6 loci visualized by the Integrative Genomics Viewer. b Pri-miR-99b-125a, miR-99b, and miR-125a-5p expression in bone marrow cells (BM), MCSF, and MCSF + IL-4-treated BMDMs. Each data point represents the mean and standard deviation (SD) of three individual animals. *P ˂ 0.05, **P ˂ 0.01. c Expression of the pri-miR-99b-125a polycistron in IL-4-stimulated or unstimulated BMDMs derived from wild-type (WT) or Stat6-defficient (Stat6 KO) mice. Each data point represents the mean and SD of three individual animals. *P ˂ 0.05. d H3K27Ac ChIP-qPCR in IL-4-treated or nontreated WT or Stat6 KO mouse BMDMs at the potential TSS of pri-miR-99b-125a. Data are expressed as mean ± SD. e RT-PCR-based detection of a common transcript of pri-miR-99b-125a and Spaca6 in IL-4-treated or nontreated WT mouse BMDMs. Cyclophilin A (cyclo) serves as normalization control