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Table 1 Patient characteristics, cfDNA concentration, and sequencing statistics

From: Next-generation sequencing diagnostics of bacteremia in septic patients

ID

Time

Sex

Age (years)

cfDNA (ng/ml plasma)

Sequencing depth

Human reads (%)

Unmapped (%)

Classified (%)

S9

T0

M

82

120.59

30,650,143

92.90

7.10

28.90

S10

T0

M

68

307.83

27,199,593

98.70

1.30

2.85

S11

T0

M

62

805.50

27,073,879

93.61

6.39

20.73

S19

T0

F

62

101.30

26,892,684

98.45

1.55

4.75

S23

T0

M

79

146.70

24,917,032

97.12

2.88

3.85

S26

T0

M

66

1088.90

32,529,889

96.60

3.40

3.24

S60

T0

F

70

70.29

27,381,853

97.10

2.90

4.40

  

Average S T0

70

377.30

28,092,153

96.36

3.64

9.82

  

Average S all

70

197.23

25,960,730

97.79

2.21

4.24

V5

 

M

24

35.80

34,203,815

81.90

18.10

12.38

V6

 

M

29

27.40

30,000,000

98.96

1.04

2.25

V7

 

F

22

76.40

21,004,601

96.58

3.42

2.35

V13

 

F

26

23.50

24,449,232

98.09

1.91

3.26

V14

 

M

28

38.60

37,971,559

97.42

2.58

1.79

V15

 

M

27

166.80

24,505,696

97.60

2.40

2.88

V16

 

F

29

70.60

27,220,925

97.06

2.94

2.67

V17

 

M

26

28.40

20,225,374

98.61

1.39

3.30

V18

 

M

28

48.80

19,157,938

98.14

1.86

2.46

V19

 

F

31

33.40

25,776,920

97.08

2.92

2.87

V21

 

M

22

67.30

25,220,391

97.72

2.28

2.51

V22

 

M

25

48.20

30,000,000

99.15

0.85

3.25

  

Average V

26

55.43

26,644,704

96.52

3.48

3.50

P1

T0

M

58

50.20

22,389,868

96.95

3.05

1.79

P2

T0

M

53

552.00

30,000,000

98.57

1.43

3.35

P3

T0

F

62

109.50

18,796,573

94.69

5.31

1.38

P4

T0

F

72

36.42

28,457,744

94.91

5.09

4.59

P5

T0

M

64

65.38

28,547,804

95.48

4.52

3.72

P7

T0

M

76

82.50

28,845,398

96.69

3.31

1.00

  

Average P T0

64

149.33

26,172,898

96.21

3.79

2.64

  

Average P T1–T2

64

451.63

25,406,269

97.72

2.28

2.14

  

Average P all

64

350.86

25,661,812

97.22

2.78

2.31

  1. Patients were grouped as septic patients (S), healthy volunteers (V), and non-infected patients following major abdominal surgery (P). Of the total reads (sequencing depth), all reads mapped to human reference genome hg19 are classified as human reads; the remaining reads are denoted as unmapped. The proportion of unmapped reads classified to any species using Kraken are specified here as classified. F female, M male