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Table 1 Patient characteristics, cfDNA concentration, and sequencing statistics

From: Next-generation sequencing diagnostics of bacteremia in septic patients

ID Time Sex Age (years) cfDNA (ng/ml plasma) Sequencing depth Human reads (%) Unmapped (%) Classified (%)
S9 T0 M 82 120.59 30,650,143 92.90 7.10 28.90
S10 T0 M 68 307.83 27,199,593 98.70 1.30 2.85
S11 T0 M 62 805.50 27,073,879 93.61 6.39 20.73
S19 T0 F 62 101.30 26,892,684 98.45 1.55 4.75
S23 T0 M 79 146.70 24,917,032 97.12 2.88 3.85
S26 T0 M 66 1088.90 32,529,889 96.60 3.40 3.24
S60 T0 F 70 70.29 27,381,853 97.10 2.90 4.40
   Average S T0 70 377.30 28,092,153 96.36 3.64 9.82
   Average S all 70 197.23 25,960,730 97.79 2.21 4.24
V5   M 24 35.80 34,203,815 81.90 18.10 12.38
V6   M 29 27.40 30,000,000 98.96 1.04 2.25
V7   F 22 76.40 21,004,601 96.58 3.42 2.35
V13   F 26 23.50 24,449,232 98.09 1.91 3.26
V14   M 28 38.60 37,971,559 97.42 2.58 1.79
V15   M 27 166.80 24,505,696 97.60 2.40 2.88
V16   F 29 70.60 27,220,925 97.06 2.94 2.67
V17   M 26 28.40 20,225,374 98.61 1.39 3.30
V18   M 28 48.80 19,157,938 98.14 1.86 2.46
V19   F 31 33.40 25,776,920 97.08 2.92 2.87
V21   M 22 67.30 25,220,391 97.72 2.28 2.51
V22   M 25 48.20 30,000,000 99.15 0.85 3.25
   Average V 26 55.43 26,644,704 96.52 3.48 3.50
P1 T0 M 58 50.20 22,389,868 96.95 3.05 1.79
P2 T0 M 53 552.00 30,000,000 98.57 1.43 3.35
P3 T0 F 62 109.50 18,796,573 94.69 5.31 1.38
P4 T0 F 72 36.42 28,457,744 94.91 5.09 4.59
P5 T0 M 64 65.38 28,547,804 95.48 4.52 3.72
P7 T0 M 76 82.50 28,845,398 96.69 3.31 1.00
   Average P T0 64 149.33 26,172,898 96.21 3.79 2.64
   Average P T1–T2 64 451.63 25,406,269 97.72 2.28 2.14
   Average P all 64 350.86 25,661,812 97.22 2.78 2.31
  1. Patients were grouped as septic patients (S), healthy volunteers (V), and non-infected patients following major abdominal surgery (P). Of the total reads (sequencing depth), all reads mapped to human reference genome hg19 are classified as human reads; the remaining reads are denoted as unmapped. The proportion of unmapped reads classified to any species using Kraken are specified here as classified. F female, M male