Fig. 5

Panx3 expression during tumorigenesis and the effect of Panx inhibitors on inflammatory interleukin expression. a Panx3 expression is plotted by tissue. Expression levels are depicted by box-and-whiskers plots, with values lying further than 1.5 times the interquartile range plotted as dots. Comparisons between all tissues are significant; Tail vs. Papillomas, p < 1e-10; Tail vs. Carcinomas p < 1e-10; Papillomas vs. Carcinomas, p < 3e-5. b Interleukin 6 (Il6) and interleukin 1b (Il1b) transcript abundances relative to control mice are plotted following TPA and/or carbenoxolone (CBX) treatment. Mice were treated with IP saline or 100 mg/kg CBX, followed by 200 μL topical acetone or TPA in acetone. Control mice were defined as mice treated with IP saline and topical acetone. Tissues were harvested 24 h after topical treatment with TPA or acetone. Expression levels are shown as ddCt values transformed to relative transcript abundances. Bars represent the 95 % CI around the mean, with the mean plotted as a square. c Tissue histology following TPA and/or CBX treatment. Representative skin samples from mice treated as described above are displayed for each treatment group. Arrows denote high areas of infiltration in representative samples from mice treated with the combination of TPA and CBX, as assessed by an observer blinded to sample IDs. Several areas showing representative levels of infiltrating cells are enlarged for the TPA/acetone and TPA/CBX treated samples