Fig. 4

a Ranking (x-axis) of melanoma (SKCM) samples according to MITF expression (gene TPM) (y-axis). We indicate the top and bottom 10 % and 25 % of the samples used for analyses. b Survival (Kaplan–Meyer) plot for the top and bottom 10 % of the samples according to the ranking of MITF expression. The p value corresponds to the Cox regression between the two groups. HR hazard ratio. c Heat map of PSI values, from 0 (blue) to 1 (yellow), for the top 30 discriminant isoforms according to |ΔPSI| value between the MITF+ and MITF– subgroups. Isoforms are labeled by gene name (y-axis). Samples are clustered according to the PSI values using Euclidean distance and Ward’s method. d Accuracy given in terms of the distribution of AUC values (y-axis) from a tenfold cross-validation for (from left to right in the x-axis) the survival model for MITF+, MITF– as well as for the separation between MITF+ and MITF– subgroups using 25 % (Q1 vs. Q4) or 10 % (D1 vs. D10) of the top and bottom samples in the ranking of MITF expression. The bars show the minimum, mean, and maximum AUC values. e Distribution of PSI values for the isoform in MADD that is predictive of prognosis in the MITF– subgroup (Wilcoxon test p value = 7.781e-05)