Table 1 Results of unbiased metagenomic deep sequencing and conventional diagnostic tests on intraocular fluid samples
From: Illuminating uveitis: metagenomic deep sequencing identifies common and rare pathogens
Subject | Clinical diagnosis | Sample type | MDS | PCR | RT- PCR | Culture | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Total culture read pairs | Percentage unique non-host read pairs | Organism (number of unique read pairs) | HSV-1 | HSV-2 | VZV | CMV | T. gondii | RV | ||||
1 | Anterior uveitis | Aqueous fluid | 16,919,211 | 0.003 | HSV-1 (423) | Pos | Neg | Neg | Neg | Neg | NA | NA |
2 | Panuveitis | Vitreous fluid | 4,551,967 | 0.10 | C. neoformans (8469) | Neg | Neg | Neg | Neg | Neg | NA | C. neoformans |
3 | Panuveitis | Vitreous fluid | 10,759,511 | 0.02 | T. gondii (1853) | Neg | Neg | Neg | Neg | Pos | NA | NA |
4 | Panuveitis (noninfectious) | Aqueous fluid | 9,548,748 | 0.01 | Neg | Neg | Neg | Neg | Neg | Neg | NA | NA |
5 | Epiretinal membrane (noninflammatory) | Vitreous fluid | 7,167,502 | 0.04 | Neg | NA | NA | NA | NA | NA | NA | NA |
6 | Anterior and intermediate uveitis | Aqueous fluid, right eye | 1,684,220 | 0.41 | RV (585) | NA | NA | NA | NA | NA | Pos | NA |
Vitreous fluid, left eye | 12,111,540 | 0.01 | RV (10) | Neg | Neg | Neg | Neg | Neg | NA | NA | ||
Control | H20 | 983,525 | 4.13 | – | NA | NA | NA | NA | NA | NA | NA | |