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Fig. 7 | Genome Medicine

Fig. 7

From: Epigenomic and metabolic responses of hypothalamic POMC neurons to gestational nicotine exposure in adult offspring

Fig. 7

PNE upregulated an antisense RNA, Gm15851, in hypothalamic POMC neurons of adult offspring. a Two-way clustering of RNA-seq expression data of POMC neurons reveal overlapping gene expression signatures in PNE offspring (N1–N6) and control offspring (C1–C5). The color code represents the gene expression value in log2CPM. Only genes with average expression levels > 1 CPM across all offspring were considered. b Strand-specific qRT-PCR confirms overexpression of Gm15851 in POMC neurons of PNE offspring (*, p < 0.05, n = 5–6, two-tailed t test with Welch correction). c Schematic of genomic localization of Gm15851 within a 28.29 kb segment spanning the chromosomal coordinates 133.9 Mb and 133.92 Mb of chromosome 1. Gm15851 is a spliced antisense transcript to Prelp and Optc on the forward strand of mouse chromosome 1. Boxes denote exons. Arrowheads denote gene orientation

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