Epigenomic and metabolic responses of hypothalamic POMC neurons to gestational nicotine exposure in adult offspring

Table 2 RNA-seq alignment statistics

Sample	Input read-pairs	Uniquely mapped read-pairs	Uniquely mapped read-pairs (%)
N1	39,371,421	28,694,853	72.9
N2	33,778,273	23,411,127	69.3
N3	37,980,952	26,573,624	70.0
N4	43,271,579	31,630,187	73.1
N5	98,021,209	67,023,419	68.4
N6	25,381,274	18,901,075	74.5
C1	43,903,675	28,076,176	63.9
C2	31,996,428	22,462,184	70.2
C3	39,669,137	26,617,656	67.1
C4	31,587,579	23,443,335	74.2
C5	46,728,455	33,418,612	71.5
Average	42,880,907	30,022,932	70.5
Average nicotine (n = 6)	46,300,785	32,705,714	71.3
Average control (n = 5)	38,777,055	26,803,593	69.4

Six PNE offspring (denoted as N1–N6) and five control offspring (denoted as C1–C5) were paired-end sequenced on Illuminas HiSeq2000 sequencer. Read-pairs passing Illumina’s internal quality filters (Input read-pairs) were aligned to the mouse mm10 reference genome using STAR [41]. Read-pairs mapped to a unique site of the mouse mm10 reference genome with less than two mismatches (Uniquely mapped read-pairs) were used for further analyses

ISSN: 1756-994X