Fig. 1
Low body weights persist following correction of chronic dietary restriction initiated at post-weaning in female mice. a Time course of normalized body weight changes from day 0 (Δbwt) representing 4 weeks of age (n = 12 mice/group). AL ad-libitum-fed cohort, LF limited-fed mice subjected to dietary restriction titrated to prevent weight gain, LF-RF limited-fed-refed mice representing a subset of LF mice given unrestricted access to food following 97 days of dietary restriction, TLF-RF a subset of LF-RF mice treated with twice daily subcutaneous injections of LONG R3 recombinant human insulin-like growth factor 1 (LONG R3 rhIGF1), a potent IGF1 analog with reduced affinity for IGF-binding proteins, from day 13 of the study. b Time period identified by gray shading in A. Vertical lines indicate feces collection. c One-week average body weight changes centered on the day of the last feces collection (days 117–123). *, P < 0.05 by Student-Newman-Keuls multiple comparison tests. n.s. not significant. d Average food intake of TLF-RF mice between days 0 and 96 expressed as the percentage of average food intake of LF mice over the same period. n.s. not significant. e Two-day average food intake determined on days 117 and 123. *, P < 0.05 by Dunn’s multiple comparison tests. n.s. not significant. LF mice weighed ~60 % less than AL controls after 167 days of dietary restriction. Body weights did not recover for at least 10 weeks after ad libitum refeeding despite comparable food intake. LONG R3 rhIGF1 facilitated body weight recovery