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Fig. 1 | Genome Medicine

Fig. 1

From: qKAT: a high-throughput qPCR method for KIR gene copy number and haplotype determination

Fig. 1

Schematic view of the KIR multiplex qPCR assay qKAT. Sequence specific primers are used for relative quantification of target KIR genes against a reference gene of fixed copy number. Each multiplex qPCR assay detects the simultaneous amplification of two target KIR genes and one reference gene. Two sets of primers target different exons of the two KIR genes and one pair of primers amplify the reference gene. Dual-labelled hydrolysis probes that are specific to each amplicon are used to monitor the PCR amplification in real-time

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