Fig. 4

Validation of CSK as a novel putative epigenetically regulated gene in HGSOC. a Mean methylation (%) ± SD of CSK in patient and F3 generation tumors in three different PDXs. b Mean methylation (%) ± SD of CSK in three different F3 generation PDX tumors treated with DAC or vehicle (PDX-36, -37 and -56, n = 3 mice in each group); **p < 0.01, ****p < 0.0001. c CSK mRNA relative expression in three different F3 generation PDX tumors treated with DAC or vehicle (PDX-36, -37 and -56, n = 3 mice in each group) using qRT-PCR; *p < 0.05. d Bisulfite pyrosequencing of CSK in a panel of ovarian cancer cell lines (n = 11), untreated and treated with DAC (1 μM) for 72 h. Mean methylation (%) ± SD of CSK for three analyzed CpG sites; **p < 0.01, ***p < 0.001, ****p < 0.0001. e qRT-PCR for CSK mRNA expression in the same ovarian cancer cell line panel, untreated and treated with DAC (1 μM) for 72 h. Relative fold induction ± SD of CSK for three independent experiments; *p < 0.05, **p < 0.01. f Correlation analysis of methylation and expression of ovarian cancer cell lines (n = 11) treated or untreated with DAC, showing an inverse correlation between methylation and expression