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Table 1 Sequencing performance for all samples

From: Copy number analysis by low coverage whole genome sequencing using ultra low-input DNA from formalin-fixed paraffin embedded tumor tissue

 

MCT6-NEB-UA

MCT6-NEB-WGA

MCT6-UT-UA

MCT6-UT-WGA

MCT6-20 ng

MCT6-5 ng

MCT4-NEB-UA

MCT4-NEB-WGA

MCT4-UT-UA

MCT4-UT-WGA

MCT4-20 ng

MCT4-5 ng

LP S1

LP S2

LP S3

LP S4

P1

P2

Mean coverage

1.34

0.14

1

0.18

1.96

2.23

1.53

0.19

1.24

0.22

2.25

2.13

2.66

1.68

1.8

2.1

1.98

0.75

Total reads (millions)

62.4

16.4

48.5

17.9

90.2

104.9

72.1

19.2

59.3

19.9

101.7

101.9

119.9

81.3

88.3

96.1

88.4

40.3

Mapped reads (millions)

61.7

14.0

47.0

15.4

88.5

102.5

71.2

15.9

57.7

17.1

101.1

100.9

119.0

80.8

87.7

95.5

87.7

39.5

Reads mapped (%)

98.96

85.57

96.97

85.85

98.04

97.66

98.73

83.18

97.35

86.17

99.4

99.1

99.28

99.39

99.29

99.32

99.23

98.09

Reads duplicates (%)

4.89

35.67

5.73

26.73

4.13

5.74

5.14

28.8

5.73

20.65

4.54

8.28

5.14

7.26

10.56

5.38

4.18

12.66

Total reads minus duplicates (millions)

59.4

11.4

45.8

13.8

86.5

99.0

68.4

14.6

55.9

16.3

97.1

93.5

113.8

75.5

79.1

90.9

84.7

35.3

Target bases (%) > = onefold coverage

58.26

9.17

48.53

11.41

67.97

71.54

61.91

11.45

56.48

14.2

74.44

72.15

80.14

62.94

65.31

73.84

72.33

33.37

Target bases (%) > = tenfold coverage

0.14

0.01

0.07

0.01

0.48

0.78

0.19

0.01

0.11

0.02

0.6

0.52

0.83

0.26

0.3

0.31

0.25

0.06

Median fragment length

124

80

120

85

111

110

113

88

124

98

120

111

124

96

101

119

122

79

Tissue age (years)

5

5

5

5

5

5

5

5

5

5

5

5

2

7

2

9

7

11

  1. NEB treated with NEB repair kit, UT untreated, UA unamplified, MCT MCC samples, LP breast tumor samples, P pre-cancerous breast lesions