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Fig. 6 | Genome Medicine

Fig. 6

From: Imiquimod has strain-dependent effects in mice and does not uniquely model human psoriasis

Fig. 6

Effects of IMQ on the expression of cell type-specific genes (Immunological Genome Project). a Heatmap colors depict average IMQ responses of 100 signature genes specifically expressed by each cell population (bottom margin; percentage of sex–strain combinations with significantly increased (red) or decreased (blue) signature gene expression; FDR <0.05, Wilcoxon rank sum test). b Replicate CD4+ and CD8+ T cell populations. Heatmap colors depict average IMQ responses of 100 signature genes for each T-cell population (left margin: n = naïve T cell, m = memory T cell; e = effector T cell; magenta font indicates representative populations shown in a and c). c CD8.7e signature genes (GEO samples GSM538387, GSM538388, and GSM605897). P values (vertical axis) quantify the degree of cell type-specific expression for each gene (CD8.7e versus other IGP cell types, Wilcoxon rank sum test). df RT-PCR analysis of Cd3g (d), Cd4 (e), and Cd8 (f) expression (n = 5 per strain/sex/treatment group; n = 40 mice total). Groups without the same letter differ significantly (P < 0.05, Tukey honest significant difference; error bars represent standard error of the mean; p values, strain-by-treatment interaction effect). Rn18s was used as an endogenous control to estimate relative gene expression. F female, FC fold change, M male, PN uninvolved skin from psoriasis patients, PP lesional skin from psoriasis patients, qPCR quantitative PCR

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