Fig. 2

Number and length of var exon 1 sequences. a Complete exon 1 sequence extracted from whole-genome assemblies using standard bioinformatic methods, requiring a minimum sequence length of 2 Kb. b Complete exon 1 sequences reconstructed from whole-genome assemblies by ETHA. Distributions of var exon 1 lengths in each sample are represented by a box-and-whiskers plot: the median is indicated by a dark line, the first and third quartiles by the boundaries of the box, and the minimum and maximum, excluding outliers, by the whiskers. Outliers are those points lying beyond the first or third quartile by more than 1.5 times the interquartile range. Clinical samples are colored by clonality (polyclonal: orange, monoclonal: blue). 3D7 is shown in gray. The number of exon 1 sequences per sample, estimated from the counts of extracted exon 1 sequences are listed on the bottom (see “Methods”)