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Fig. 3 | Genome Medicine

Fig. 3

From: PathOS: a decision support system for reporting high throughput sequencing of cancers in clinical diagnostic laboratories

Fig. 3

Quality control of runs and samples. Screen shots of graphical quality control metrics. Quality control is monitored at the sample, sequencing run and amplicon level. a A sequencing run’s read yield is compared to all previous runs of the same assay and should reside between ± 2 standard deviations for the last ten runs. Failed runs can be seen here dropping below the lower bound. b All samples within a run can be compared and samples with below average reads are highlighted in red. c The per amplicon reads over all samples in the run are binned and graphed to highlight their distribution and highlight any amplicons with less than 100 reads. Non-template controls are included in each run and are flagged if they contain any reads. Both a sequencing run and samples within the run must be QC passed or failed by the user prior to curation reports being produced. d The configurable heatmap of number of reads by amplicon and sample. Lighter horizontal bands indicate poorly performing amplicons while lighter vertical bars show poorly sequenced samples, typically due to insufficient or fragmented sample DNA

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