Fig. 1

The detectable tryptic space of reference and variant human proteins. a Distribution of 2.9 million reference proteome tryptic peptides (length 6–35 amino-acids; including two possible trypsin missed cleavages) derived from four commonly used reference proteomes. Counts are represented using a log₁₀ scale. Panels (b–f) use the prostate cancer cell-line PC-3 as an example. b Distribution of the 35,445 variant peptides that are also contained within at least one reference proteome. Y-axis covariate depicts the source of the variant. Color gradient indicates the percentage of the 35,446 variants that overlap with each reference using a log₁₀ scale. c Numbers of protein variants in the nine major database variants used to search PC-3 proteomics data. Counts are in a log₁₀ scale. d Total number of exome-seq derived variant peptides and their membership in other databases. Counts are in a log₁₀ scale. e Total number of RNA-seq derived variant peptides and their membership in other databases. Counts are in a log₁₀ scale. f Total number of peptides derived from various community-based databases and their redundancy with each other. Counts are in a log₁₀ scale