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Fig. 1 | Genome Medicine

Fig. 1

From: A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications

Fig. 1

General workflow of single-cell RNA-sequencing (scRNA-seq) experiments. A typical scRNA-seq workflow includes most of the following steps: 1) isolation of single cells, 2) cell lysis while preserving mRNA, 3) mRNA capture, 4) reverse transcription of primed RNA into complementary DNA (cDNA), 5) cDNA amplification, 6) preparation of cDNA sequencing library, 7) pooling of sequence libraries, 8) use of bio-informatic tools to assess quality and variability, and 9) use of specialized tools to analyse and present the data. t-SNE t-distributed stochastic neighbour embedding

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