Table 1 Brief overview of scRNA-seq approaches
From: A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications
Protocol example | C1 (SMARTer) | Smart-seq2 | MATQ-seq | MARS-seq | CEL-seq | Drop-seq | InDrop | Chromium | SEQ-well | SPLIT-seq |
|---|---|---|---|---|---|---|---|---|---|---|
Transcript data | Full length | Full length | Full length | 3’-end counting | 3’-end counting | 3’-end counting | 3’-end counting | 3’-end counting | 3’-end counting | 3’-end counting |
Platform | Microfluidics | Plate-based | Plate-based | Plate-based | Plate-based | Droplet | Droplet | Droplet | Nanowell array | Plate-based |
Throughput (number of cells) | 102–103 | 102–103 | 102–103 | 102–103 | 102–103 | 103–104 | 103–104 | 103–104 | 103–104 | 103–105 |
Typical read depth (per cell) | 106 | 106 | 106 | 104–105 | 104–105 | 104–105 | 104–105 | 104–105 | 104–105 | 104 |
Reaction volume | Nanoliter | Microliter | Microliter | Microliter | Nanoliter | Nanoliter | Nanoliter | Nanoliter | Nanoliter | Microliter |
Reference | [63] | [57] | [39] | [10] | [64] | [45] | [46] | [47] | [101] | [38] |