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Table 1 Brief overview of scRNA-seq approaches

From: A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications

Protocol example C1 (SMARTer) Smart-seq2 MATQ-seq MARS-seq CEL-seq Drop-seq InDrop Chromium SEQ-well SPLIT-seq
Transcript data Full length Full length Full length 3’-end counting 3’-end counting 3’-end counting 3’-end counting 3’-end counting 3’-end counting 3’-end counting
Platform Microfluidics Plate-based Plate-based Plate-based Plate-based Droplet Droplet Droplet Nanowell array Plate-based
Throughput (number of cells) 102–103 102–103 102–103 102–103 102–103 103–104 103–104 103–104 103–104 103–105
Typical read depth (per cell) 106 106 106 104–105 104–105 104–105 104–105 104–105 104–105 104
Reaction volume Nanoliter Microliter Microliter Microliter Nanoliter Nanoliter Nanoliter Nanoliter Nanoliter Microliter
Reference [63] [57] [39] [10] [64] [45] [46] [47] [101] [38]