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Fig. 1 | Genome Medicine

Fig. 1

From: Cancer gene profiling in non-small cell lung cancers reveals activating mutations in JAK2 and JAK3 with therapeutic implications

Fig. 1

Examples for the detection of JAK2 p.V617F mutation by targeted NGS and real-time allele-specific PCR. a Targeted NGS: IGV view for JAK2 c.1849G > T (p.V617F) mutation in sample 1825. The sequencing depth at the mutation site is 1998x, with 268 sequencing reads representing the mutant allele (T) and 1730 reads representing the WT allele (G). b Real-time allele-specific PCR on LightCycler 480 instrument (Roche). An example for one sample shown in the amplification plot: a. results for JAK2 WT; b. results for JAK2 c.1849G > T (p.V617F). The rest of the curves represent unrelated samples in the PCR reaction

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