Fig. 1

DNA labeling for NGM. The DNA labeling workflow is divided into four consecutive steps. First, the high molecular weight DNA is nicked with an endonuclease of choice that introduces single strand nicks throughout the genome. Second, Taq polymerase recognizes these sites and replaces several nucleotides with fluorescently tagged nucleotides added to the solution. Third, the two ends of the DNA are ligated together using DNA ligase. Fourth, the DNA backbone is stained with DNA Stain