Fig. 2

Irys/Saphyr chip nanochannel structure and DNA loading. The labeled dsDNA is loaded into two flowcells of either Irys or Saphyr chips. The applied voltage concentrates the coiled DNA at the lip (left). Later, DNA is pushed through pillars (middle) to uncoil/straighten, then into nanochannels (right). DNA is stopped and imaged in the nanochannels. Blue = staining of DNA backbone, green = fluorescently labeled nicked sites