Fig. 1
From: Methylation patterns in serum DNA for early identification of disseminated breast cancer
Study design. Using reduced representation bisulfite sequencing (RRBS), 31 human tissue samples were analyzed to identify a total of 18 regions which underwent thorough technical validation. Six regions were selected whose methylation status has been analyzed in two sets consisting of 110 serum samples. One marker (EFC#93) has been validated in two independent settings: (1) in SUCCESS study serum samples from BC patients before and after chemotherapy; and (2) in UKCTOCS serum samples from women before BC diagnosis (within three years) or who remained healthy for five years