Fig. 2
From: Methylation patterns in serum DNA for early identification of disseminated breast cancer
Principles of methylation pattern discovery in tissue (a, b) and analyses in serum (c). a RRBS was used in tissue samples in order to identify CpG methylation patterns that are able to discriminate breast cancer from white blood cells (which were deemed to be the most abundant source of cell-free DNA). “0” represents an unmethylated CpG and “1” represents a methylated CpG. An example of region EFC#93 is provided which is a 136-bp-long region containing five linked CpGs. The cancer pattern consists of reads in which all linked CpGs are methylated, indicated by “11111.” b RRBS data have been processed through a bioinformatic pipeline to identify the most promising markers. c The principles of the serum DNA methylation assay