Fig. 2From: Linking FOXO3, NCOA3, and TCF7L2 to Ras pathway phenotypes through a genome-wide forward genetic screen in human colorectal cancer cellsThe transposon target genes FOXO3, NCOA3, and TCF7L2 modulate the Ras pathway phenotype of growth in low glucose and regulate GLUT1 expression. Real-time growth curves for stable shRNA knockdown lines of NCOA3, FOXO3, and TCF7L2 in DLD-1 KRASwt/- cells in low-glucose medium (a) and normal medium (b). GLUT1 expression level after stable knockdown of these three genes in DLD-1 KRASwt/- (c, d) and RKO BRAFwt/-/- (e, f). Gene expression levels were measured by qPCR using beta-actin as endogenous control and normalized to shControl. Each experiment was performed three times with three technical replicates. The statistical analysis was performed by Student’s t-test where ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05Back to article page