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Fig. 2 | Genome Medicine

Fig. 2

From: Linking FOXO3, NCOA3, and TCF7L2 to Ras pathway phenotypes through a genome-wide forward genetic screen in human colorectal cancer cells

Fig. 2

The transposon target genes FOXO3, NCOA3, and TCF7L2 modulate the Ras pathway phenotype of growth in low glucose and regulate GLUT1 expression. Real-time growth curves for stable shRNA knockdown lines of NCOA3, FOXO3, and TCF7L2 in DLD-1 KRASwt/- cells in low-glucose medium (a) and normal medium (b). GLUT1 expression level after stable knockdown of these three genes in DLD-1 KRASwt/- (c, d) and RKO BRAFwt/-/- (e, f). Gene expression levels were measured by qPCR using beta-actin as endogenous control and normalized to shControl. Each experiment was performed three times with three technical replicates. The statistical analysis was performed by Student’s t-test where ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05

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