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Fig. 3 | Genome Medicine

Fig. 3

From: Linking FOXO3, NCOA3, and TCF7L2 to Ras pathway phenotypes through a genome-wide forward genetic screen in human colorectal cancer cells

Fig. 3

Modulation of the Ras pathway phenotypes of MEK/ERK phosphorylation and anchorage-independent growth by FOXO3, NCOA3, and TCF7L2. Soft agar assays (a) and growth curves in normal medium (b) in stable shRNA lines of NCOA3, FOXO3, and TCF7L2 in DLD-1 cells (KRAS G13D). The impact of NCOA3, FOXO3, and TCF7L2 perturbation on pMEK and pERK in relation to MEK and ERK, respectively, were quantified by NanoPro analysis in DLD-1 (KRAS G13D) (c, e) and RKO (BRAF V600E) (d, f) cells with shKRAS and shBRAF as positive controls. Each experiment was performed at least twice with three technical replicates with statistical analysis by Student’s t-test where ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05

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Genome Medicine

ISSN: 1756-994X

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