Fig. 2
Establishment of transgenic flies co-expressing TREM2WT or TREM2R47H with TYROBP in glial cells. a mRNA expression of TREM2 or TYROBP driven by a pan-glial driver Repo-LexA was confirmed by RT-PCR analysis. b Western blotting of fly head expressing TREM2WT or TYROBP driven by Repo-LexA. TREM2 and TYROBP were tagged with Myc-DDK (FLAG). Membranes were probed with anti-FLAG antibody. Control; Repo-LexA driver alone. c Western blotting of fly head co-expressing TREM2 with TYROBP driven by Repo-LexA. Membranes were probed with anti-TREM2 or anti-TYROBP antibody. Tubulin was used as a loading control. No significant difference. (Student’s t-test). d TYROBP co-immunoprecipitated with TREM2. Fly head lysates were subjected to immunoprecipitation with anti-TYROBP antibody, followed by western blotting with anti-TYROBP or anti-TREM2 antibody. Top: Western blotting of crude lysate. Bottom: Immunoprecipitate with anti-TYROBP antibody was subjected to western blotting. e Western blotting of fly head lysate co-expressing TREM2 and TYROBP by anti-TREM2 antibody detected a full length of TREM2 as well as the C-terminal fragment of TREM2 (TREM2-CTF). Genotypes of flies are described in Additional file 2: Table S1