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Fig. 5 | Genome Medicine

Fig. 5

From: Integrated biology approach reveals molecular and pathological interactions among Alzheimer’s Aβ42, Tau, TREM2, and TYROBP in Drosophila models

Fig. 5

Gene regulatory network analysis of gene expression signatures in Aβ42/TREM2/TYROBP flies with human AD WGCNA. a Overlap between HBTRC or ROSMAP human AD WGCNA co-expression network modules and DEGs identified in (Fig. 3a and Additional file 1: Figure S2C). b mRNA expression levels of genes from “synaptic transmission” module validated by qPCR. mRNA levels in the heads of flies with neuronal expression of Aβ42 alone (Aβ42), glial expression of TREM2R47H/TYROBP (TREM2R47H/TYROBP) alone and neuronal expression of Aβ42 and glial expression of TREM2R47H/TYROBP (Aβ42/TREM2R47H/TYROBP) were analyzed by qRT-PCR. Control; drivers alone. Mean ± SEM, n = 4, *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with post-hoc Tukey’s test. c Neuronal knockdown of para worsened Aβ42-induced locomotor deficits as revealed by climbing assay. d Neuronal knockdown of para by itself caused modest decline in locomotor functions upon aging. Mean ± SEM, n = 3–5, *p < 0.05 and **p < 0.01 by Student’s t-test. Genotypes of flies are described in Additional file 2: Table S1

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